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Cell Culture 6

Cell line initiation, maintenance and passaging Freezing/thawing and cell bank creation Viability counting and contamination diagnosis/troubleshooting Gene silencing (siRNA/RNAi) Organoid culture Live-cell imaging (JULI)

Cytotoxicity and Biocompatibility 4

Single-dose MTT viability assay Dose–response series and IC50 calculation (4PL curve fitting; 24/48/72 hours) Multi-drug / multi-cell-line panel ISO 10993-5/-12 extract-based in vitro cytotoxicity (dental, aligner, biomedical products)

Oxidative Stress and DNA Damage 2

ROS measurement (DCF-DA) DNA damage: γH2AX immunofluorescence

Tissue Homogenisation 4

Tissue homogenisation Protein lysate preparation and quantification Tissue lysis for RNA/DNA isolation ELISA measurement from homogenate

Tissue Processing and Paraffin Embedding 5

Tissue fixation Tissue processing (dehydration, clearing, paraffin infiltration) Paraffin embedding Microtome sectioning and slide preparation Block and slide archiving/delivery

Immunofluorescence Staining 5

Cell (culture) immunofluorescence Paraffin tissue section immunofluorescence (including deparaffinisation and antigen retrieval) Antibody optimisation Double/multiple staining Imaging and fluorescence intensity quantification

Immunohistochemistry and Tissue Analysis 3

Routine histological staining and evaluation IHC staining Scoring and comparative evaluation report

Nucleic Acid and PCR 4

RNA/DNA isolation and purity check cDNA synthesis Primer design and validation (Primer-BLAST, OligoAnalyzer, in silico PCR) RT-qPCR and relative expression analysis (ΔΔCt)

ELISA 2

ELISA measurement and standard curve Dilution factor calculation and result interpretation

Bacterial Culture and Analysis 1

Bacterial culture and analysis

Project and Ethics Committee Consultancy 4

Experimental design and project writing (TÜBİTAK, TÜSEB, KOSGEB, BAP) Ethics committee dossier and application management (including TİTCK amendment procedures) Power analysis and material/cost planning Invention disclosure and patent specification support

Data Analysis and Publication Support 4

Statistical analysis Publication-quality figures and schematics Final report (BAP final report format) Thesis/article writing support and reference verification